LOCALIZATION OF HLA-ABC AND DR ANTIGENS IN HUMAN KIDNEY : Transplantation (original) (raw)

TRANSPLANTATION ARTICLE: PDF Only

HART, DEREK N. J.; FUGGLE, SUSAN V.; WILLIAMS, KERYN A.; FABRE, JOHN W.; TING, ALAN; MORRIS, PETER J.

Nuffield Department of Surgery, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, England

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Abstract

Monoclonal antibodies to human monomorphic class I and class II major histocompatibility complex (MHC) determinants have been used with immunofluorescence and immunoperoxidase techniques, to localize these antigens in normal human kidneys. HLA-DR antigen was located in the glomeruli (probably on endothelium as well as in the mesangium) and within the cells of cortical and medullary tubules. Dendritic cells in the renal inter-stitium stained brightly for the DR antigen and could be distinguished from the staining of capillary endothelium. The vascular endothelium of large vessels stained less densely for the HLA-DR antigen than for HLA-ABC antigens. The glomeruli stained intensely for the HLA-ABC antigens and diffuse staining of HLA-ABC antigens was also noted within renal tubular cells.