Lactobacillus plantarum 299V in the Treatment and Prevention of Spontaneous Colitis in Interleukin-10-Deficient Mice (original) (raw)

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*Center for GI Biology and Disease, Department of Medicine, and

†Department of Internal Medicine I, University of Regensburg, Germany;

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*Center for GI Biology and Disease, Department of Medicine, and

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*Center for GI Biology and Disease, Department of Medicine, and

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†Department of Internal Medicine I, University of Regensburg, Germany;

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‡College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, U.S.A.

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†Department of Internal Medicine I, University of Regensburg, Germany;

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*Center for GI Biology and Disease, Department of Medicine, and

§Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina, U.S.A.;

Address correspondence and reprint requests to R. Balfour Sartor, M.D., Division of Digestive Diseases and Nutrition, School of Medicine, CB#7038, Room 032 Glaxo Building, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7038, U.S.A.

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Accepted:

06 September 2001

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Michael Schultz, Claudia Veltkamp, Levinus A. Dieleman, Wetonia B. Grenther, Pricilla B. Wyrick, Susan L. Tonkonogy, R. Balfour Sartor, Lactobacillus plantarum 299V in the Treatment and Prevention of Spontaneous Colitis in Interleukin-10-Deficient Mice, Inflammatory Bowel Diseases, Volume 8, Issue 2, 1 March 2002, Pages 71–80, https://doi.org/10.1097/00054725-200203000-00001
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Summary

Interleukin (IL)-10-deficient (IL-10−/−) mice develop colitis under specific pathogen-free (SPF) conditions and remain disease free if kept sterile (germ free [GF]). We used four different protocols that varied the time-points of oral administration of Lactobacillus plantarum 299v (L. plantarum) relative to colonization with SPF bacteria to determine whether L. plantarum could prevent and treat colitis induced by SPF bacteria in IL-10−/− mice and evaluated the effect of this probiotic organism on mucosal immune activation. Assessment of colitis included blinded histologic scores, measurements of secreted colonic immunoglobulin isotypes, IL-12 (p40 subunit), and interferon (IFN)-γ production by anti-CD3-stimulated mesenteric lymph node cells. Treating SPF IL-10−/− mice with L. plantarum attenuated previously established colonic inflammation as manifested by decreased mucosal IL-12, IFN-γ, and immunoglobulin G2a levels. Colonizing GF animals with L. plantarum and SPF flora simultaneously had no protective effects. Gnotobiotic IL-10−/− mice monoassociated with L. plantarum exhibited mild immune system activation but no colitis. Pretreatment of GF mice by colonization with L. plantarum, then exposure to SPF flora and continued probiotic therapy significantly decreased histologic colitis scores. These results demonstrate that L. plantarum can attenuate immune-mediated colitis and suggest a potential therapeutic role for this agent in clinical inflammatory bowel diseases.

© 2002 Crohn's & Colitis Foundation of America, Inc.

Topic:

• inflammation
• colitis
• interleukin-10
• interleukin-12
• interleukins
• lactobacillus
• bacteria
• colon
• mice
• microbial colonization
• prevention

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