Monocyte chemoattractant protein-1 immunoreactivity in... : NeuroReport (original) (raw)

SOMATOSENSORY SYSTEMS, PAIN

Monocyte chemoattractant protein-1 immunoreactivity in sensory ganglia and hindpaw after adjuvant injection

Jeon, Sang-Mina; Lee, Kyung-Mina; Park, Eun-Sungb; Jeon, Young-Hoonc; Cho, Hee-Junga

aDepartment of Anatomy, School of Medicine

bDepartment of Oral Biochemistry, School of Dentistry, Kyungpook National University

cDepartment of Anesthesiology and Pain Medicine, Kyungpook National University Hospital, Daegu, South Korea

Correspondence to Hee-Jung Cho, Department of Anatomy, School of Medicine, Kyungpook National University, 2-101, Dongin Dong, Daegu, 700-422, South Korea

Tel: +(053)420 6922; fax: +(053)427 1468; e-mail: [email protected]

Received 23 October 2007; accepted 26 October 2007

Abstract

Monocyte chemoattractant protein-1 (MCP-1)/CCL2 is a member of the CC chemokine family that exhibits potent chemotactic activity for monocytes/macrophages. In the current study, the proportion of monocyte chemoattractant protein-1-immunoreactive (IR) neurons in the dorsal root ganglion (DRG) of rats was shown to increase markedly following adjuvant injection into the hindpaw. MCP-1-IR axon terminals were not found in the spinal cord or hindpaw of control or adjuvant-treated rats. Instead, the inflamed hindpaw dermis was infiltrated by numerous MCP-1-IR inflammatory cells. Following adjuvant injection, the majority of MCP-1-IR neurons in the DRG colocalized with IB4 binding. Our findings suggest that peripheral tissue inflammation induces increased MCP-1 expression in DRG neurons and this may be dependent upon glial cell line-derived neurotrophic factor.