GABA and glycine frequently colocalize in terminals on cat... : NeuroReport (original) (raw)
Original Articles: PDF Only
Department of Anatomy, Erasmus University Medical School, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands
Abstract
In this ultrastructural study the colocalization of γ-amino butyric acid (GABA) and glycine in terminals within cat lumbar motoneuronal cell groups was investigated and the frequency of this colocalization was determined. For this purpose the post-embedding immunogold technique was applied on serial sections, using antibodies directed against either GABA or glycine. Analysis of all labelled terminals in a random area of cat motoneuronal cell groups showed that 25 ± 5% were labelled for GABA only, 29 ± 6% were labelled for glycine only and 46 ± 9% were labelled for both GABA and glycine, meaning that nearly two out of every three GABA-labelled terminals were also labelled for glycine and vice versa. Based on these results and on other data suggesting a high frequency of colocalization, it is concluded that in cat motoneuronal cell groups colocalization of GABA and glycine is the rule rather than the exception.
© Lippincott-Raven Publishers.