Opposite changes in adenosine A1 and A2A receptor mRNA in... : NeuroReport (original) (raw)

Sleep

Opposite changes in adenosine A1 and A2A receptor mRNA in the rat following sleep deprivation

Basheer, Radhika1,* CA; Halldner, Linda2*; Alanko, Lauri3; McCarley, Robert W.1; Fredholm, Bertil B.2; Porkka-Heiskanen, Tarja1,3

1Harvard Medical School and V.A. Medical Center, Department of Psychiatry, Rm 2B126, Bldg 3, West Roxbury, MA 02132, USA; 2Department of Physiology and Pharmacology, Karolinska Institute, S-171 77 Stockholm, Sweden; 3Institute of Biomedicine, University of Helsinki, Helsinki, Finland

CA,4Corresponding Author and Address *Contributed equally to this work

Received 14 February 2001; accepted 16 March 2001

Abstract

Extracellular levels of adenosine increase in basal forebrain following prolonged wakefulness. Moreover, perfusion of adenosine into basal forebrain increases sleep. In this study we have examined the adenosine receptor subtypes, A1 and A2A, for changes in the levels of mRNA using RT-PCR and in situ hybridization and the receptor ligand binding efficiency using autoradiography following 3 and 6 h of sleep deprivation. We observed that A1 receptor mRNA levels increased in basal forebrain with no changes in other forebrain areas examined. A1 receptor binding was not affected. A2A receptor mRNA and ligand binding were undetectable in basal forebrain. However, in the olfactory tubercle, A2A mRNA and receptor binding decreased significantly. Based on the significant increase in the A1 but not in A2A receptor, we hypothesize that the effects of sleep deprivation-induced increased adenosine are mediated by A1 receptor in basal forebrain of rats.