Induction of IgG Antibodies by an Anti-Idiotype Antibody... : Journal of Immunotherapy (original) (raw)
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Induction of IgG Antibodies by an Anti-Idiotype Antibody Mimicking Disialoganglioside GD2
Sen, Goutam; Chakraborty, Mala; Foon, Kenneth A.; Reisfeld, Ralph A.*; Bhattacharya-Chatterjee, Malaya
Department of Medicine and Markey Cancer Center, University of Kentucky, Lexington, Kentucky
* The Scripps Research Institute, La Jolla, California, U.S.A
Abstract
Summary:
The anti-idiotype (Id) monoclonal antibody (mAb) 1A7 immunoglobulin Gl (IgGl, K), raised in syngeneic mice against the murine anti-ganglioside GD2 mAb 14G2a mimics a carbohydrate epitope on GD2 and serves as a surrogate protein antigen for this disialoganglioside. Immunization of allogeneic C57BL/6 mice and rabbits with 1A7 induced anti-GD2 antibodies of IgG isotype that recognize purified GD2 by enzyme-linked immunosorbent assay (ELISA) and GD2-positive human melanoma cells (M21/P6) by fluorescence-activated cell sorter (FACS) analysis. The specificity of the antisera for GD2 was further confirmed by dot-blot analysis. These antisera also specifically lyse GD2-positive M2l/P6 target cells in an antibodydependent cellular cytotoxicity assay. Taken together, these results suggest that the anti-Id 1A7 can induce GD2-specific IgG antibodies that can recognize cell surfaceassociated as well as soluble disialoganglioside GD2.
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