Autocrine Growth Effect of IL-8 and GROα on a Human... : Pancreas (original) (raw)

Articles

Autocrine Growth Effect of IL-8 and GROα on a Human Pancreatic Cancer Cell Line, Capan-1

Takamori, Hiroshi; Oades, Zenaida G.*; Hoch, Robert C.*; Burger, Meike†; Schraufstatter, Ingrid U.†

First Department of Surgery, Kumamoto University School of Medicine, Kumamoto, Japan; *Department of Immunology, The Scripps Research Institute, and †La Jolla Institute for Experimental Medicine, La Jolla, California, U.S.A.

Manuscript received August 19, 1999;

revised manuscript accepted November 16, 1999.

Address correspondence and reprint requests to Dr. H. Takamori, First Department of Surgery, Kumamoto University School of Medicine, 1-1-1 Honjo, Kumamoto 860-8556, Japan. E-mail: takamori@ mail.kuh.kamamoto-u.ac.jp

Abstract

A human pancreatic cancer cell line, Capan-1, secretes the chemokines interleukin-8 (IL-8) and growth-related oncogene alpha (GROα). Capan-1 cells also express the chemokine receptor 2 (CXCR2), which is a Giα-protein coupled receptor. Growth of Capan-1 cells was inhibited when anti-IL-8 or anti-GROα monoclonal antibody was added into the culture medium. Pertussis toxin, which blocks Giα also demonstrated a growth-inhibitory effect on Capan-1 cells. These results indicated that IL-8 and GROα act on Capan-1 cells as growth factors in an autocrine manner through CXCR2.