A NEW MICROMETHOD FOR THE DETECTION OF HL-A ANTIGENS ON... : Transplantation (original) (raw)

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A NEW MICROMETHOD FOR THE DETECTION OF HL-A ANTIGENS ON CULTURED HUMAN TUMOR CELLS

FRITZE, DIETER; KERN, DAVID H.; DROGEMULLER, CAROL R.; PILCH, YOSEF H.

Department of Surgery, Harbor General Hospital, Torrance, California 90509, and the UCLA School of Medicine, Los Angeles, California 90024

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Abstract

A rapid microcytotoxicity assay for the detection of HL-A antigens on tissue culture cells derived from human solid tumors is described. Tumor cells were prelabeled with 125Iododeoxyuridine. Isotopically labeled tumor cells were reacted with up to 37 highly selected HL-A antisera and diluted rabbit complement. Results of the HL-A typing of nine human tumor cell lines are reported. Three melanoma cell lines showed individually distinct HL-A profiles at the first HL-A locus which agreed with the antigenic pattern of the tumor donor's autologous lymphocytes. Less reactivity was noted with HL-A antisera defining second locus specificities on the three melanoma cell lines, whereas some other cell lines showed more HL-A reactions than required to present a “full house.” This method obviates the necessity for visually enumerating residual tumor target cells.