HIV and SIV Envelope Glycoproteins Induce Phospholipase A2... : JAIDS Journal of Acquired Immune Deficiency Syndromes (original) (raw)

Basic Science

HIV and SIV Envelope Glycoproteins Induce Phospholipase A2 Activation in Human and Macaque Lymphocytes

Mavoungou, Elie*†; Georges-Courbot, Marie-Claude†; Poaty-Mavoungou, Virginie†; Nguyen, Hahn T.†; Yaba, Philippe*; Delicat, André*; Georges, Alain J.*†; Russo-Marie, Françoise‡

Departments of *Immunology and Immunogenetics and †Retrovirology, International Center for Medical Research, Franceville, Gabon, and ‡Institut Cochin de Génétique Moléculaire, INSERM U332, Paris, France

Address correspondence and reprint requests to Dr. E. Mavoungou, Centre International de Recherches Médicales de Franceville (CIRMF), B.P. 769, Franceville, Gabon;[email protected].

Manuscript received December 5, 1996; accepted April 4, 1997.

Abstract

Summary

We investigated the early interactions between HIV-1, HIV-2, and simian immunodeficiency virus (SIV) envelope glycoproteins gp120IIIB, gp105ROD, and gp120mac251, and human and macaque cells of the lymphocytic series. Our results demonstrate that the soluble viral glycoproteins induce a specific phospholipase A2(PLA2) activation in lymphocytes through CD4. This PLA2 activation was induced after envelope glycoprotein-CD4 interaction and, because of its local membrane-destabilizing effect, may have important implications for preparing the lymphocyte membrane for fusion with the viral particle. However, this effect is not sufficient to accomplish fusion. These data indicate that the specific step of fusion may be downstream from PLA2 activation.