Static Magnetic Fields Up-regulate Osteoblast Maturity by... : Clinical Orthopaedics and Related Research® (original) (raw)

SECTION II: ORIGINAL ARTICLES: Research

Static Magnetic Fields Up-regulate Osteoblast Maturity by Affecting Local Differentiation Factors

Huang, Haw-Ming MS*†; Lee, Sheng-Yang DDS, PhD‡; Yao, Wei-Cheng MD§; Lin, Che-Tong DDS, PhD*; Yeh, Ching-Ying PhD†

From the *Graduate Institute of Oral Sciences; the †Graduate Institute of Medical Sciences; the ‡School of Dentistry, Taipei Medical University, Taipei, Taiwan; and the §Department of Anesthesiology, Min-Sheng General Hospital, Taoyuan, Taiwan.

Received: April 12, 2005

Revised: August 23, 2005; October 20, 2005; November 23, 2005

Accepted: November 23, 2005

The first author (H-M H) has received funding from a grant (93MSH-TMU- 01) from Min-Sheng General Hospital, Taoyuan, Taiwan, and by a grant (NSC- 92-2320-038-001) from the National Science Council, Taipei, Taiwan.

Correspondence to: Ching-Ying Yeh, PhD, Graduate Institute of Medical Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei, Taiwan. Phone: 886-2-2736-1661 ext. 6515; Fax: 886-2-87326789; E-mail: [email protected].

Clinical Orthopaedics and Related Research 447():p 201-208, June 2006. | DOI: 10.1097/01.blo.0000203464.35561.be

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Abstract

Cell culture studies have shown that static magnetic fields induce osteoblastic differentiation at an early stage. However, the mechanisms of differentiated effects have not been well described. We postulated that static magnetic fields stimulate osteoblastic differentiation by regulating early local factors released by the cells. To examine our hypothesis, MG63 osteoblast-like cells were exposed continuously to 0.4-T static magnetic fields for 12, 24, 48, and 72 hours. The morphologic changes and matrix vesicles release were observed by scanning and transmission electron microscopy. The effects of static magnetic fields on levels of transforming growth factor-β1, Type I collagen, osteopontin, and alkaline phosphatase were compared between the exposed and unex- posed cells. The data suggest MG63 cells treated with static magnetic fields have more differentiated morphologic features. The local regulatory factors produced by static magnetic field-treated cells were greater than those of the control cultures. These findings provide evidence that static magnetic fields affect osteoblastic maturation by up-regulating early local factors.

© 2006 Lippincott Williams & Wilkins, Inc.