Downregulation of miR-125b in metastatic cutaneous... : Melanoma Research (original) (raw)

ORIGINAL ARTICLES: Translational research

Glud, Martina e; Rossing, Mariab; Hother, Christofferc; Holst, Linee; Hastrup, Ninad; Nielsen, Finn C.b f; Gniadecki, Roberte f; Drzewiecki, Krzysztof T.a f

Departments of aPlastic Surgery and Burn Unit

bClinical Biochemistry

cHaematology, Epigenomisk Laboratory

dPathology, Rigshospitalet

eDermatology, Bispebjerg Hospital

fFaculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark

Correspondence to Martin Glud, MD, Department of Plastic Surgery and Burn Unit, Rigshospitalet, University of Copenhagen, Blegdamsvej 9, Copenhagen 2100, Denmark

Tel: +45 2762215; fax: +45 35316010; e-mail: [email protected]

Received 20 May 2010 Accepted 7 July 2010

Abstract

This study aimed to identify microRNA species involved in the earliest metastatic event in cutaneous malignant melanoma (MM). Samples from 28 patients with MM [stage T2 (tumor), M0 (distant metastasis)] were grouped by the presence of micrometastasis in the sentinel lymph nodes (N0/N1). Melanoma cells were harvested from primary, cutaneous MM tumors by laser-capture microdissection, and microRNA expression profiles were obtained by the microarray technique. Results were validated by quantitative reverse transcription PCR. We found that miR-125b was downregulated in the primary cutaneous melanomas that produced early metastases (T2, N1, M0) compared with the sentinel lymph node-negative (T2, N0, M0) melanomas. MiR-125b has earlier been found to be downregulated in other tumor types and in atypic naevi compared with the common acquired naevi. In conclusion, miR-125b may be involved in an early progression of cutaneous MM.