Effects of Glycyrrhizin on the Differentiation of Myeloid... : Shock (original) (raw)

Basic Science Aspects

Effects of Glycyrrhizin on the Differentiation of Myeloid Cells of the Heart and Lungs in Lipopolysaccharide-Induced Septic Mice

Seo, Eun-Hye*; Song, Ga-Yun†; Kwak, Byung Ok†; Oh, Chung-Sik‡; Lee, Seung Hyun†; Kim, Seong-Hyop‡,§

*Department of Cellular and Molecular Medicine, Konkuk University School of Medicine, Seoul, Korea

†Department of Microbiology, Konkuk University School of Medicine, Seoul, Korea

‡Department of Anesthesiology and Pain medicine, Konkuk University Medical Center, Konkuk University School of Medicine, Seoul, Korea

§Department of Medicine, Institute of Biomedical Science and Technology, Konkuk University School of Medicine, Seoul, Korea

Address reprint requests to Seong-Hyop Kim, MD, PhD, Department of Anesthesiology and Pain Medicine, Konkuk University Medical Center, Konkuk University School of Medicine, Neudong-ro (Hwayang-dong), Gwangjin-gu, Seoul 05030, Korea. E-mail: [email protected]

Received 17 January, 2017

Revised 1 February, 2017

Accepted 9 February, 2017

This paper was supported by Konkuk University.

The roles of the authors: all authors read and accepted terms and conditions of Shock. E-HS: data collection, data analysis and interpretation, manuscript composition. G-YS: data collection, data analysis and interpretation. BOK:

data analysis and interpretation. C-SO: data analysis and interpretation. SHL:

data analysis and interpretation. S-HK: study design, data collection, data analysis and interpretation, manuscript composition.

The authors report no conflicts of interest.

Abstract

Background:

This study investigated the effects of glycyrrhizin (GR) on the ratio of myeloid-derived suppressor cells (MDSCs) to cluster of differentiation (CD)11b+Gr1 myeloid cells in the heart and lungs in lipopolysaccharide (LPS)-induced septic mice.

Methods:

Mice were divided into three groups: Control, LPS, and LPS+GR. After intraperitoneal injection of phosphate-buffered saline for the Control group, LPS for the LPS group, and a combination of LPS and GR for the LPS+GR group, fluorescence-activated cell sorting was utilized to evaluate cytokines and immune cells in the blood, heart, and lungs. Histopathologic analysis of Toll-like receptor (TLR)4 was also performed.

Results:

The cytokine amounts in the LPS and LPS+GR groups were significantly higher than in the Control group; however, that in the LPS+GR group was significant lower than in the LPS group. The ratio of MDSCs to CD11b+Gr1 myeloid cells in the LPS+GR group was significantly higher than in the LPS group but was significantly lower than in the Control group. The staining intensity of TLR4 showed the same pattern as that of cytokines in the heart and lungs. TLR4 staining was significantly lower in the LPS+GR group than in the LPS group but was higher than that in the Control group.

Conclusion:

GR exhibited protective effects on the heart and lungs in LPS-induced septic mice. The effects were associated with an elevated ratio of MDSCs to CD11b+Gr1 myeloid cells and the inhibition of cytokine release and TLR4 expression after GR injection.