Structure and function of antifreeze proteins (original) (raw)
Research article| 29 Jul 2002
1
Department of Biochemistry
,
Queen's University, Kingston, Ontario, Canada K7L 3N6
2
Department of Biology
,
Queen's University, Kingston, Ontario, Canada K7L 3N6
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1
Department of Biochemistry
,
Queen's University, Kingston, Ontario, Canada K7L 3N6
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2
Department of Biology
,
Queen's University, Kingston, Ontario, Canada K7L 3N6
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2
Department of Biology
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Queen's University, Kingston, Ontario, Canada K7L 3N6
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Publisher: The Royal Society
Online ISSN: 1471-2970
Print ISSN: 0962-8436
Philos Trans R Soc Lond B Biol Sci (2002) 357 (1423): 927–935 .
Abstract
High–resolution three–dimensional structures are now available for four of seven non–homologous fish and insect antifreeze proteins (AFPs). For each of these structures, the ice–binding site of the AFP has been defined by site–directed mutagenesis, and ice etching has indicated that the ice surface is bound by the AFP. A comparison of these extremely diverse ice–binding proteins shows that they have the following attributes in common. The binding sites are relatively flat and engage a substantial proportion of the protein's surface area in ice binding. They are also somewhat hydrophobic—more so than that portion of the protein exposed to the solvent. Surface–surface complementarity appears to be the key to tight binding in which the contribution of hydrogen bonding seems to be secondary to van der Waals contacts.
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