Early Alteration of Poliovirus in Infected Cells and Its Specific Inhibition (original) (raw)

Abstract

SUMMARY

HeLa cells infected with radioactive poliovirus type 2 were disrupted with ultrasonic treatment, followed by addition of a non-ionic detergent. Two types of virus particles were found to sediment at 80 to 90% the rate of native virus. The first of these appeared to be a complex of native virus particles and membrane components, since treatment with 0.2% SDS released infectious native particles. The second was non-infectious and its sedimentation rate was not greatly altered by SDS. One hour after infection this non-infectious particle was the major product of cell-mediated eclipse.

We have confirmed that 10 to 30 μg/ml S-7, a substituted thiopyrimidine, blocks infection of cells by poliovirus in a specific manner. Analysis of cells infected with radioactive poliovirus type 2 in the presence of S-7 showed that the virus particles remained as the complex which can be disrupted with SDS. In addition to blocking cell-mediated eclipse, S-7 stabilizes poliovirus against heat inactivation in vitro at the same concentrations which block infection. This action resembles the effect of 10−2 M-glutathione, which is also known to block cell-mediated eclipse of poliovirus.

Received: 21/11/1974
Accepted:29/01/1975
Published Online:01/06/1975

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-27-3-329

1975-06-01

2024-11-18

Full text loading...

/deliver/fulltext/jgv/27/3/JV0270030329.html?itemId=/content/journal/jgv/10.1099/0022-1317-27-3-329&mimeType=html&fmt=ahah