Early and Delayed Shut-off of Host Protein Synthesis in Cells Infected with Herpes Simplex Virus (original) (raw)

Abstract

SUMMARY

A mutant of herpes simplex virus type 1 [HSV- 1(HFEM)], tsB7, appears to have two temperature-sensitive functions. One is required during the first hour of infecting a cell (suggesting that it is performed by a virion protein) and the other is the non-essential function of ‘early shut-off’ of cellular protein synthesis, which is mediated by a virion protein. The latter function remained temperature-sensitive in a revertant virus (RC2) grown at the non-permissive temperature (39 °C). However, under these conditions RC2 did cause inhibition of host synthesis, showing that ‘delayed shut-off’, requiring virus protein synthesis, can occur independently of early shut-off, which is mediated by a virion protein. Early shut-off by u.v.-irradiated tsB7 was reversed when the temperature was raised, whereas delayed shut-off by intact tsB7 was not. Of two wild-type strains of virus examined, HSV-1 (F) also exhibited temperature-sensitive early shut-off, but HSV-2(G) did not.

Received: 23/11/1981
Accepted:25/02/1982
Published Online:01/07/1982

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-61-1-121

1982-07-01

2024-10-22

Full text loading...

/deliver/fulltext/jgv/61/1/JV0610010121.html?itemId=/content/journal/jgv/10.1099/0022-1317-61-1-121&mimeType=html&fmt=ahah