125I-labelled Human Interferons Alpha, Beta and Gamma: Comparative Receptor-binding Data (original) (raw)

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Abstract

SUMMARY

Binding of 125I-labelled human recombinant DNA interferons (IFNs) alpha-2, beta and gamma was compared on various human lymphoid cells and embryonic fibroblasts. While binding constants were within an order of magnitude for all three interferons (10−10 to 10−9 m), no competition was observed between IFN-γ on the one hand and IFN-α2 and IFN-β on the other. However, consistent with previous reports, IFN-α2 and IFN-β competed for presumably common receptors. Depending on the cell type, binding sites for IFN-γ were expressed in different numbers compared to those for IFN-α2 and IFN-β. These direct comparative binding studies support the hypothesis that the receptor system for IFN-γ is unrelated to the IFN-α/β system.

• Received: 23/11/1984
• Accepted:22/01/1985
• Published Online:01/05/1985

© Journal of General Virology 1985

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1985-05-01

2025-01-12

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