The human cytomegalovirus 86K immediate early (IE) 2 protein requires the basic region of the TATA-box binding protein (TBP) for binding, and interacts with TBP and transcription factor TFIIB via regions of IE2 required for transcriptional regulation (original) (raw)

• _info_Info
  • Article information
  • Full-Text
  • Figures and Tables
  • References (37)
  • Cited By (132)
  • Supplementary Material
  • Metrics
• _list_Sections

Abstract

The 86K immediate early (IE) 2 protein of human cytomegalovirus trans-activates a number of homologous and heterologous promoters, including the cellular promoter for the 70K heat-shock protein (hsp 70), and the human immunodeficiency virus long terminal repeat. We have previously shown that IE2 trans-activates these two promoters in a TATA-dependent manner, and that IE2 is able to form a direct contact with TATA-box binding protein (TBP) in vitro. We now show that IE2 binds to the basic repeat region of TBP. In addition IE2 can contact a second general transcription factor, TFIIB. We have mapped the TBP- and TFIIB-binding regions within IE2 and show that these regions overlap, and also lie within parts of the protein previously identified as being required for the trans-activation and autoregulation functions of IE2.

• Received: 07/06/1993
• Accepted:11/08/1993
• Published Online:01/12/1993

© Journal of General Virology 1993

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-12-2691

1993-12-01

2024-10-23

Full text loading...

/deliver/fulltext/jgv/74/12/JV0740122691.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-12-2691&mimeType=html&fmt=ahah

Most read this month

Article

content/journal/jgv

Journal

5

3

false

en