Expression, identification and subcellular localization of the proteins encoded by the hepatitis C viral genome (original) (raw)

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Abstract

We have expressed the full-length coding region and selected domains of the hepatitis C virus (HCV) cDNA in mammalian cells by transfection. Using HCV antibody-positive human sera and monospecific antibodies the proteins encoded by the putative structural and non-structural regions of the open reading frame of HCV were identified as core (p22), E1 (gp32–35), E2 (gp68–72), NS2 (p23), NS3 (p72), NS4a and b (p10 and p27) and NS5a and b (p56 and p70). We have also defined the subcellular localizations of the HCV proteins using indirect immunofluorescence assays.

• Received: 28/10/1992
• Accepted:29/01/1993
• Published Online:01/06/1993

© Society for General Microbiology 1993

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1993-06-01

2025-01-01

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