Biochemical Comparison of Pili from Variants of Neisseria gonorrhoeae P9 (original) (raw)
Abstract
Four different types of pili produced by variants of Neisseria gonorrhoeae P9 were isolated and characterized. The pili differed in subunit molecular weight with SDS-PAGE and in subunit isoelectric point on agarose gels. Isoelectric points of the major molecular species in Tritonagarose gels of octylglucoside solubilized pili were: δ, pI 6·5; α;, pI 6·0; β, pI 5·3 and γ, pI 5·5. Amino acid analyses of pili showed close homology between different types but a reduction in the content of aspartate and serine was notable in the low molecular weight δ pili; also β and γ pili contained more alanine residues. Structural homology was also demonstrated in peptide maps of tryptic/chymotryptic digests of pili with several major peptides apparently common to all four pilus types.
- Received: 01/12/1981
- Revised:19/02/1982
- Published Online:01/09/1982
© Society for General Microbiology 1982
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1982-09-01
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