Over-production and Characterization of the nifA Gene Product of Klebsiella pneumoniae — the Transcriptional Activator of nif Gene Expression (original) (raw)

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Abstract

The nifA gene of Klebsiella pneumoniae, which encodes the transcriptional activator of nif gene expression, was cloned into a number of plasmid vectors to obtain high-level synthesis of nifA product (NifA). When over-produced, NifA was very insoluble and it precipitated with the cell debris after cell lysis. Localization of β-galactosidase activity from a nifA-lacZ translational fusion confirmed the insoluble nature of NifA. Analysis of two translational fusions in which the last six C-terminal amino acids of NifA were deleted suggests that these residues are required for activity.

• Received: 14/09/1987
• Published Online:01/02/1988

© Society for General Microbiology, 1988

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1988-02-01

2025-01-15

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