A Simple Chemically Defined Medium for the Production of Phase I Bordetella pertussis (original) (raw)

Abstract

SUMMARY: A simple, chemically defined medium is described consisting of sodium glutamate, proline, cystine, salts, and growth factors, which is suitable for the large-scale production of phase I Bordetella pertussis. More than 30 × 109 organisms/ml. were produced in 48 to 72 h. growth in shake flasks and fer- mentors. The cultures were detoxified by the addition of 0·14 % formalin to yield vaccines which were non-toxic to mice and guinea pigs and had good mouse-protective antigen levels. The antigenic stability was satisfactory after storage of the final vaccines at elevated temperatures.

Received: 09/07/1970
Published Online:01/10/1970

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/content/journal/micro/10.1099/00221287-63-2-211

1970-10-01

2024-11-20

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