Sequence variability of FrpB, a major iron-regulated outer-membrane protein in the pathogenic neisseriae (original) (raw)

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Abstract

The FrpB protein from pathogenic neisseriae is a 77 kDa iron-regulated outer-membrane protein that belongs to the family of TonB-dependent receptors and may have potential as a vaccine component. Comparison between the frpB gene from three different meningococcal strains and a published gonococcal one revealed that the region from residues 350 to 390 displays pronounced sequence variability. In a model for the topology of FrpB in the outer membrane, this region corresponds to loop 7, the longest of the predicted 13 surface-exposed loops. Binding of four out of a total of eight bactericidal monoclonal antibodies to synthetic peptides corresponding to loop 7 showed that their epitopes are located here. The frpB genes from five additional meningococcal strains were cloned and sequenced in this region. Pairwise comparisons showed different degrees of similarity.

• Received: 15/04/1996
• Accepted:15/07/1996
• Revised:09/07/1996
• Published Online:01/11/1996

©Society for General Microbiology 1996

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1996-11-01

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