Dialister succinatiphilus sp. nov. and Barnesiella intestinihominis sp. nov., isolated from human faeces (original) (raw)

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Abstract

Two anaerobic, non-spore-forming, bacteria (YIT 11850T and YIT 11860T) that stained Gram-negative, were isolated from human faeces. Cells of strain YIT 11850T were coccobacilli, asaccharolytic and largely unreactive, with only traces of lactate and propionate as metabolic end products; however, strain YIT 11850T was able to decarboxylate succinate to propionate. The DNA G+C content of strain YIT 11850T was 51.9 mol%. Following 16S rRNA gene sequence analysis, this strain was found to be most closely related to Dialister propionicifaciens, with 95.1 % sequence similarity between the two taxa. Biochemical data supported the affiliation of strain YIT 11850T to the genus Dialister. Strain YIT 11850T therefore represents a novel species for which the name Dialister succinatiphilus sp. nov. is proposed; the type strain is YIT 11850T (=DSM 21274T=JCM 15077T). Cells of the other isolate, strain YIT 11860T, were non-motile, rod-shaped, positive for aesculin hydrolysis, negative for indole production, produced succinic and acetic acids as end products of glucose metabolism and possessed a DNA G+C content of 45.5 mol%. On the basis of 16S rRNA gene sequence similarity values, this strain was shown to belong to the family ‘Porphyromonadaceae’ related to Barnesiella viscericola (96.0 %); similarity values with species within the family ‘Porphyromonadaceae’ with validly published names were less than 86 %. Biochemical data supported the affiliation of strain YIT 11860T to the genus Barnesiella. Strain YIT 11860T therefore represents a novel species for which the name Barnesiella intestinihominis sp. nov. is proposed; the type strain is YIT 11860T (=DSM 21032T=JCM 15079T).

• Published Online:01/12/2008

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2008-12-01

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