The in vivo ϕX mRNA (original) (raw)

1. John W. Sedat and
2. Robert L. Sinsheimer
3. Division of Biology, California Institute of Technology, Pasadena, California

Excerpt

In recent years extensive studies have been made concerning the replication of ϕX RF (Knippers, Whalley, and Sinsheimer, 1969), ϕX viral DNA (Knippers, Razin, Davis, and Sinsheimer, 1969), and the ϕX specific proteins synthesized during infection (Burgess and Denhardt, 1969; Gelfand and Hayashi, 1969; Mayol and Sinsheimer, unpubl.). In contrast, ϕX messenger RNA (mRNA) has received scant experimental attention since the initial observation of Hayashi, Hayashi, and Spiegelman (1963) that the in vivo ϕX mRNA was transcribed off the complementary strand of the RF and the report of an in vivo pulse-labeled RNase-, phenol-, and detergent-resistant RNA-RF complex (Hayashi and Hayashi, 1966).

The process of replication, transcription, and protein synthesis would seem to be intricately related in this infection since the parental RF must be transcribed before replication, and especially since the association of RF with a replicative site (Yarus and Sinsheimer, 1967) may be requirement for participation in genetic...