The Globin Gene: Structure and Expression (original) (raw)
- J. Paul,
- R. S. Gilmour,
- N. Affara,
- G. Birnie,
- P. Harrison,
- A. Hell,
- S. Humphries,
- J. Windass, and
- B. Young
- The Beatson Institute for Cancer Research, 132 Hill Street, Glasgow Scotland
Excerpt
The complexity of the eukaryotic genome makes problems concerned with the regulation of transcription and translation particularly intractable. An obvious strategy is to concentrate on the study of a single genetic locus or, perhaps better, a very small number of coordinately regulated loci. Of several possibilities, we chose to investigate hemoglobin synthesis because of the extensive background of chemical, genetic, and cytological information that exists. Following the success in isolating the messenger RNA for globin (Chantrenne et al., 1967; Schapira et al., 1968; Benz and Forget, 1971; Lingrel et al, 1971; Williamson et al., 1971) and the discovery that a DNA copy (cDNA) of globin mRNA can be obtained with RNA-dependent DNA polymerase from avian myelo-blastosis virus (reverse transcriptase) (Ross et al., 1972b; Verma et al., 1972; Kacian and Spiegelman, 1972), it has become possible to study the globin gene and regulation of its expression in some detail.