Studies on Simian Virus 40 Excision from Cellular Chromosomes (original) (raw)

1. M. Botchan,
2. W. Topp, and
3. J. Sambrook
4. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724

Excerpt

Rodent cells in culture which do not support SV40 replication can be stably transformed by the virus. The resulting cells, which invariably harbor integrated viral DNA sequences (Sambrook et al. 1968; Hirai et al. 1971; Gelb et al. 1971), often can be manipulated to become infectious centers of viral production by fusion with permissive simian cells (Gerber 1966; Watkins and Dulbecco 1967; Koprowski et al. 1967). The source of the progeny viral DNA molecules is the integrated copy (see Discussion). This is remarkable in view of the fact that previous work from our laboratory and others has shown that integrated SV40 DNA can be attached to chromosomal DNA at various positions on the viral genome and also that multiple sites in cellular DNA are available for this viral insertion (Botchan et al. 1976; Ketner and Kelly 1976; Kucherlapati et al. 1978). Although it will be necessary to clone the inserted...