Cooperation between C/EBPα TBP/TFIIB and SWI/SNF recruiting domains is required for adipocyte differentiation (original) (raw)

  1. Thomas Åskov Pedersen1,3,
  2. Elisabeth Kowenz-Leutz2,3,
  3. Achim Leutz2,4, and
  4. Claus Nerlov1,4
  5. 1Laboratory of Gene Therapy Research, Copenhagen University Hospital, 2100 Copenhagen, Denmark; 2Max-Delbrück-Center for Molecular Medicine, 13125 Berlin, Germany

Abstract

Chromatin remodeling is an important step in promoter activation during cellular lineage commitment and differentiation. We show that the ability of the C/EBPα transcription factor to direct adipocyte differentiation of uncommitted fibroblast precursors and to activate SWI/SNF-dependent myeloid-specific genes depends on a domain, C/EBPα transactivation element III (TE-III), that binds the SWI/SNF chromatin remodeling complex. TE-III collaborates with C/EBPα TBP/TFIIB interaction motifs during induction of adipogenesis and adipocyte-specific gene expression. These results indicate that C/EBPα acts as a lineage-instructive transcription factor through SWI/SNF-dependent modification of the chromatin structure of lineage-specific genes, followed by direct promoter activation via recruitment of the basal transcription–initiation complex, and provide a mechanism by which C/EBPα can mediate differentiation along multiple cellular lineages.

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