Identification and characterization of yUAP/Sub2p, a yeast homolog of the essential human pre-mRNA splicing factor hUAP56 (original) (raw)

1. Meng Zhang and
2. Michael R. Green1
3. Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA

Abstract

The human 56-kD U2AF65-associated protein (hUAP56), a member of the DExD/H box protein family of RNA-dependent ATPases, is required for the stable binding of U2 snRNP to the pre-mRNA branchpoint. Here we identify a highly conserved Saccharomyces cerevisiae homolog of hUAP56, yUAP/Sub2p. yUAP/Sub2p can be functionally substituted for by hUAP56 and, like its human counterpart, is an essential pre-mRNA splicing factor. yUAP/Sub2p is required for formation of the commitment complex, the precursor for U2 snRNP addition. In conjunction with previous studies, we conclude that at least two DExD/H box proteins, Prp5p and yUAP/Sub2p, mediate the U2 snRNP-branchpoint interaction.

• pre-mRNA splicing
• hUAp56
• SUB2
• U2AF
• DEAD box protein
• U2 snRNP binding
• commitment complex

Footnotes

• ↵1 Corresponding author.

• E-MAIL michael.green{at}umassmed.edu; FAX (508) 856-5473.

• Article and publication are at www.genesdev.org/cgi/doi/10.1101/gad.851701.

• • Received September 18, 2000.
  • Accepted November 14, 2000.

• Cold Spring Harbor Laboratory Press

•