Saccharomyces Rrm3p, a 5′ to 3′ DNA helicase that promotes replication fork progression through telomeric and subtelomeric DNA (original) (raw)

1. Andreas S. Ivessa1,
2. Jin-Qiu Zhou1,2,
3. Vince P. Schulz,
4. Ellen K. Monson, and
5. Virginia A. Zakian3
6. Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544-1014, USA

Abstract

In wild-type Saccharomyces cerevisiae, replication forks slowed during their passage through telomeric C1–3A/TG1–3 tracts. This slowing was greatly exacerbated in the absence of RRM3, shown here to encode a 5′ to 3′ DNA helicase. Rrm3p-dependent fork progression was seen at a modified Chromosome VII-L telomere, at the natural X-bearing Chromosome III-L telomere, and at Y‘-bearing telomeres. Loss of Rrm3p also resulted in replication fork pausing at specific sites in subtelomeric DNA, such as at inactive replication origins, and at internal tracts of C1–3A/TG1–3 DNA. The ATPase/helicase activity of Rrm3p was required for its role in telomeric and subtelomeric DNA replication. Because Rrm3p was telomere-associated in vivo, it likely has a direct role in telomere replication.

• Telomere
• helicase
• telomerase
• replication
• RRM3
• yeast

Footnotes

• ↵1 These authors contributed equally to this work.

• ↵2 Present address: Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, P.R. China.

• ↵3 Corresponding author.

• E-MAIL vzakian{at}molbio.princeton.edu; FAX (609) 258-1701.

• Article and publication are at http://www.genesdev.org/cgi/doi/10.1101/gad.982902.

• • Received February 7, 2002.
  • Accepted April 10, 2002.

• Cold Spring Harbor Laboratory Press

•