Systematic Discovery of New Genes in the Saccharomyces cerevisiae Genome (original) (raw)

1. Marco M. Kessler,
2. Qiandong Zeng,
3. Sarah Hogan,
4. Robin Cook,
5. Arturo J. Morales1, and
6. Guillaume Cottarel
7. Genome Therapeutics Corporation, Waltham, Massachusetts 02453, USA

Abstract

We used genome-wide comparative analysis of predicted protein sequences to identify many novel small genes, named smORFs forsmall open readingframes, within the budding yeast genome. Further analysis of 117 of these new genes showed that 84 are transcribed. We extended our analysis of one smORF conserved from yeast to human. This investigation provides an updated and comprehensive annotation of the yeast genome, validates additional concepts in the study of genomes in silico, and increases the expected numbers of coding sequences in a genome with the corresponding impact on future functional genomics and proteomics studies.

Footnotes

• ↵1 Corresponding author.

• E-MAIL arturo.morales{at}genomecorp.com; FAX (781) 398-2476.

• Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.232903.

• • Received March 1, 2002.
  • Accepted November 7, 2002.

• Cold Spring Harbor Laboratory Press

•