Construction of a YAC contig encompassing the Usher syndrome type 1C and familial hyperinsulinism loci on chromosome 11p14-15.1. (original) (raw)

  1. R Ayyagari,
  2. A Nestorowicz,
  3. Y Li,
  4. S Chandrasekharappa,
  5. C Chinault,
  6. P van Tuinen,
  7. R J Smith,
  8. J F Hejtmancik, and
  9. M A Permutt
  10. National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.

Abstract

The Usher syndrome type 1C (USH1C) and familial hyperinsulinism (HI) loci have been assigned to chromosome 11p14-15.1, within the interval D11S419-D11S1310. We have constructed a yeast artificial chromosome (YAC) contig, extending from D11S926 to D11S899, which encompasses the critical regions for both USH1C and HI and spans an estimated genetic distance of approximately 4 cM. A minimal set of six YAC clones constitute the contig, with another 22 YACs confirming the order of sequence-tagged sites (STSs) and position of YACs on the contig. A total of 40 STSs, including 10 new STSs generated from YAC insert-end sequences and inter-Alu PCR products, were used to order the clones within the contig. This physical map provides a resource for identification of gene transcripts associated with USH1C, HI, and other genetic disorders that map to the D11S926-D11S899 interval.

Footnotes