Structural Biology of RNA Silencing and Its Functional Implications (original) (raw)

  1. D.J. PATEL*,
  2. J.-B. MA*,
  3. Y.-R. YUAN*,‡,
  4. K. YE*,,
  5. Y. PEI†,
  6. V. KURYAVYI*,
  7. L. MALININA*,§,
  8. G. MEISTER†,**, and
  9. T. TUSCHL†
  10. *Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021
  11. †Howard Hughes Medical Institute, Laboratory of RNA Biology, The Rockefeller University, New York, New York 1002
  12. ‡Present addresses: Structural Biology Group, Temasek Life Sciences Laboratory, National University of Singapore, Singapore, 117604
  13. §Present addresses: National Institute of Biological Sciences, 7 Science Park Road, Zhongguancun Life Science Park, Beijing, 102206, China
  14. ¶Present addresses: CIC biGune, Technological Park of Bizkaia, 48160 Derio, Spain
  15. **Present addresses:Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsreid, Germany.

Abstract

We outline structure–function contributions from our laboratories on protein–RNA recognition events that monitor siRNAlength, 5′-phosphate and 2-nucleotide 3′ overhangs, as well as the architecture of Argonaute, its externally bound siRNA complex,and Argonaute-based models involving guide-strand-mediated mRNA binding, cleavage, and release.

Footnotes