Recruitment of the SWI/SNF chromatin remodeling complex by transcriptional activators (original) (raw)

1. Natalya Yudkovsky,
2. Colin Logie,
3. Steven Hahn, and
4. Craig L. Peterson
5. Molecular and Cellular Biology Program, University of Washington, The Fred Hutchinson Cancer Research Center; Fred Hutchinson Cancer Research Center and the Howard Hughes Medical Institute, Seattle Washington 98109 USA; Program in Molecular Medicine and Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605 USA

Abstract

SWI/SNF is a chromatin remodeling complex that facilitates expression of a number of yeast genes. Here we demonstrate that SWI/SNF can be recruited from yeast nuclear extracts by a transcriptional activator. Recruitment is dependent on an activation domain but not on promoter sequences, TBP, or RNA polymerase II holoenzyme. We also show that acidic activation domains can target SWI/SNF remodeling activity. These results demonstrate that SWI/SNF activity can be targeted by gene-specific activators and that this recruitment can occur independently of Pol II holoenzyme.

• SWI-SNF
• chromatin
• transcription
• gene regulation
• holoenzyme

Footnotes

• ↵These two authors contributed equally to this work.

• ↵Corresponding author.

• E-MAIL craig.peterson{at}umassmed.edu; FAX (508) 856-4289.

• • Received June 29, 1999.
  • Accepted August 6, 1999.

• Cold Spring Harbor Laboratory Press

•