Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila (original) (raw)

  1. Hirotsugu Ishizu1,3,
  2. Miharu Komai1,
  3. Hazuki Kotani1,
  4. Yoshinori Kawamura1,
  5. Kazumichi M. Nishida1,
  6. Haruhiko Siomi1,5 and
  7. Mikiko C. Siomi1,2,4
  8. 1Keio University School of Medicine, Tokyo 160-8582, Japan;
  9. 2Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Saitama 332-0012, Japan
  10. ↵3 These authors contributed equally to this work.

Abstract

PIWI-interacting RNAs (piRNAs) protect genome integrity from transposons. In Drosophila ovarian somas, primary piRNAs are produced and loaded onto Piwi. Here, we describe roles for the cytoplasmic Yb body components Armitage and Yb in somatic primary piRNA biogenesis. Armitage binds to Piwi and is required for localizing Piwi into Yb bodies. Without Armitage or Yb, Piwi is freed from the piRNAs and does not enter the nucleus. Thus, piRNA loading is required for Piwi nuclear entry. We propose that a functional Piwi–piRNA complex is formed and inspected in Yb bodies before its nuclear entry to exert transposon silencing.

Footnotes