kuzbanian-mediated cleavage of Drosophila Notch (original) (raw)

1. Toby Lieber,
2. Simon Kidd, and
3. Michael W. Young1
4. Laboratory of Genetics, The Rockefeller University, New York, New York 10021, USA

Abstract

Loss of Kuzbanian, a member of the ADAM family of metalloproteases, produces neurogenic phenotypes in Drosophila. It has been suggested that this results from a requirement for_kuzbanian_-mediated cleavage of the Notch ligand Delta. Using transgenic Drosophila expressing transmembrane Notch proteins, we show that kuzbanian, independent of any role in Delta processing, is required for the cleavage of Notch. We show that Kuzbanian can physically associate with Notch and that removal of_kuzbanian_ activity by RNA-mediated interference in_Drosophila_ tissue culture cells eliminates processing of ligand-independent transmembrane Notch molecules. Our data suggest that in_Drosophila_, kuzbanian can mediate S2 cleavage of Notch.

• Notch
• Kuzbanian
• processing
• Drosophila

Footnotes

• ↵1 Corresponding author.

• E-MAIL young{at}mail.rockefeller.edu; FAX (212) 327-8695.

• Article and publication are at http://www.genesdev.org/cgi/doi/10.1101/gad.942302.

• • Received August 31, 2001.
  • Accepted November 26, 2001.

• Cold Spring Harbor Laboratory Press

•