X chromosome map at 75-kb STS resolution, revealing extremes of recombination and GC content. (original) (raw)

  1. R Nagaraja,
  2. S MacMillan,
  3. J Kere,
  4. C Jones,
  5. S Griffin,
  6. M Schmatz,
  7. J Terrell,
  8. M Shomaker,
  9. C Jermak,
  10. C Hott,
  11. M Masisi,
  12. S Mumm,
  13. A Srivastava,
  14. G Pilia,
  15. T Featherstone,
  16. R Mazzarella,
  17. S Kesterson,
  18. B McCauley,
  19. B Railey,
  20. F Burough,
  21. V Nowotny,
  22. M D'Urso,
  23. D States,
  24. B Brownstein, and
  25. D Schlessinger
  26. Center for Genetics in Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA.

Abstract

A YAC/STS map of the X chromosome has reached an inter-STS resolution of 75 kb. The map density is sufficient to provide YACs or other large-insert clones that are cross-validated as sequencing substrates across the chromosome. Marker density also permits estimates of regional gene content and a detailed comparison of genetic and physical map distances. Five regions are detected with relatively high G + C, correlated with gene richness; and a 17-Mb region with very low recombination is revealed between the Xq13.3 [XIST] and Xq21.3 XY homology loci.

Footnotes