Isolation and Characterization of Individual Clones of Simian Virus 40 Mutants Containing Deletions, Duplications and Insertions in Their DNA (original) (raw)

1. J. E. Mertz,
2. J. Carbon,
3. M. Herzberg,
4. R. W. Davis, and
5. P. Berg
6. Department of Biochemistry, Stanford University Medical Center, Stanford, California 94305

Excerpt

Several laboratories (Tegtmeyer et al. 1970; Robb and Martin 1970; Kit et al. 1970; Kimura and Dulbecco 1972; Chou and Martin 1974) have isolated and partially characterized conditional-lethal, temperature-sensitive (ts) mutants of simian virus 40 (SV40). These mutants have provided much valuable information about the physiology and molecular biology of the viral life cycle (Tegtmeyer and Ozer 1971; Tegtmeyer 1972 and this volume; Robb and Martin 1972; Eckhart 1974; R. Martin et al. and Lai and Nathans, this volume). However, the utility of temperature-sensitive mutants is generally limited to genes that code for proteins and by the extent to which the mutant proteins function at the restrictive temperature (i.e., their leakiness). Furthermore, the identification of altered temperature-sensitive proteins and the accurate mapping of the location of the corresponding mutations on the genome also present difficulties.

Our efforts toward the genetic analysis of SV40 have dealt with a different class of...