Chi (original) (raw)

  1. D. K. Chattoraj,
  2. J. M. Crasemann,
  3. N. Dower,
  4. D. Faulds,
  5. P. Faulds,
  6. R. E. Malone,
  7. F. W. Stahl, and
  8. M. M. Stahl
  9. Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403

Excerpt

Special sites involved in generalized recombination were signaled in the early 1960s. In Ascobolus, Lissouba (1961) showed a “polarity” in gene conversion (i.e., non-4:4 segregation in individual asci) for sites within a gene. In a set of interallelic crosses involving sites two at a time, asci containing the rare wild-type spores were usually a result of conversion of the site on one particular side. Apparently there was some factor near the gene which influenced conversion rates in its neighborhood.

Factors influencing recombination locally were revealed also by the experiments of Murray (1963) in Neurospora. Her interallelic crosses had markers flanking the close, recombining sites. She found, as previously noted by Pritchard (1960), that many (about half) of the interallelic recombinants were parental for the flanking markers (“localized negative interference”). Surprisingly, the two parental configurations had very unequal frequencies even though the flanking markers were about symmetrically placed around the close,...