Structure and Function of the Transforming Genes of Human Adenoviruses and SV40 (original) (raw)
- A. J. van der Eb,
- H. van Ormondt,
- P. I. Schrier,
- J. H. Lupker,
- H. Jochemsen,
- P. J. van den Elsen,
- R. J. DeLeys,
- J. Maat,
- C. P. van Beveren†,
- R. Dijkema, and
- A. de Waard
- Sylvius Laboratories, Department of Physiological Chemistry, University of Leiden, 2333 AL Leiden, The Netherlands
Excerpt
Transformation by human adenoviruses is a process in which only a small fraction of the viral genome is involved. This is most clearly shown by the observation that specific DNA fragments originating from the left-hand end of the genome are able to transform cells in vitro (Graham et al. 1975; van der Eb et al. 1977; van der Eb and Houweling 1977). This is further supported by the finding that rodent cells transformed by human adenovirus types 2 or 5 (Ad2 or Ad5) all contain viral DNA sequences homologous to the left-hand 14% of the genome, whereas some of the lines also contain sequences homologous to other parts of the viral DNA (Gallimore et al. 1974; Sharp et al. 1975; Flint et al. 1976). This indicates that transformation is basically a function of early region 1 (E1), which maps between 1% and 11% in the DNA of all human adenoviral...