Homologous Recombination between Repeated Chromosomal Sequences in Mouse Cells (original) (raw)

1. R.M. Liskay,
2. J.L. Stachelek, and
3. A. Letsou
4. Departments of Therapeutic Radiology and Human Genetics, Yale University School of Medicine, New Haven, Connecticut 06510

Excerpt

Until recently, only viral systems have been useful for homologous recombination studies in mammalian cells (e.g., Wake and Wilson 1979; Volkert and Young 1983). Analysis of recombination of chromosomal genes has been hindered, in part, by a lack of appropriate genetic and molecular markers. The techniques of gene transfer and gene manipulation have now enabled investigators working on mammalian cells to study homologous recombination between other types of introduced DNA molecules (Folger et al. 1982), including selectable markers (de Saint Vincent and Wahl 1983; Shapira et al. 1983; Small and Scangos 1983). There are, however, some obvious disadvantages to such gene transfer approaches. First, because one is often dealing with populations of molecules, it is difficult to identify precisely the partners in any particular recombination and, therefore, it is difficult to define the types of events taking place. Second, the timing of these events (i.e., before or after integration into...