Tandem Arrangement of Genes Coding for Tumor Necrosis Factor (TNF-α) and Lymphotoxin (TNF-β) in the Human Genome (original) (raw)

  1. S.A. Nedospasov*,
  2. A.N. Shakhov*,
  3. R.L. Turetskaya*,
  4. V.A. Mett*,
  5. M.M. Azizov*,
  6. G.P. Georgiev*,
  7. V.G. Korobko†,
  8. V.N. Dobrynin†,
  9. S.A. Filippov†,
  10. N.S. Bystrov†,
  11. E.F. Boldyreva†,
  12. S.A. Chuvpilo†,
  13. A.M. Chumakov†,
  14. L.N. Shingarova†, and
  15. Y. A. Ovchinnikov†
  16. *Institute of Molecular Biology and † Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow, USSR

Excerpt

Tumor necrosis factor and lymphotoxin, also termed TNF-α: and TNF-β (Nedwin et al. 1985a), are polypeptides secreted by peripheral blood leukocytes (PBLs). Their production is induced by mitogens, phorbol esters, or interleukin 2 (Haranaka et al. 1984; Chroboczek Kelker et al. 1985; Nedwin et al. 1985a). Both factors are highly cytotoxic to a wide range of tumor cells in vitro and in vivo (for review, see Ruff and Gifford 1981; Old 1985; Sugarman et al. 1985). It appears that TNF-α: and TNF-β are either the only or the major cytotoxic proteins present in the crude lymphokine preparations from stimulated PBLs (Chroboczek Kelker et al. 1985). Apparently, both proteins bind to the same receptor molecules on the surfaces of normal and tumor cells (Aggarwal et al. 1985a). Although they originate from different types of cells, TNF-α: and TNF-β share 30% amino acid homology and show similar cytostatic and cytolytic activities in...