Targeting of Cytoplasmic Dynein to Membranous Organelles and Kinetochores via Dynactin (original) (raw)

1. R.B. Vallee1,
2. K.T. Vaughan1, and
3. C.J. Escheverri1,2
4. 1Worcester Foundation for Biomedical Research (formerly Worcester Foundation for Experimental Biology), Shrewsbury Massachusetts 01545; 2Department of Cell Biology, University of Massachusetts Graduate School of Biomedical Sciences, Worcester, Massachusetts 01605

Excerpt

Cytoplasmic dynein is a motor protein responsible for movement along microtubules toward their minus ends (Paschal and Vallee 1987). On the basis of its direction of force production, the rate of in vitro microtubule gliding motility it supports, and its enzymatic properties, we proposed that cytoplasmic dynein mediates retrograde axonal transport and other forms of minus-end-directed organelle movement (Paschal and Vallee 1987; Vallee et al. 1989a). A variety of pharmacological, immunocytochemical, and in vitro motility data have supported such a role (for review, see Porter and Johnson 1989; Vallee and Shpetner 1990; Holzbaur and Vallee 1994). Cytoplasmic dynein has been specifically implicated in mediating the movement and specifying the distribution of late endosomes and lysosomes (Lin and Collins 1992), Golgi elements (Corthesy-Theulaz et al. 1992), early to late endosomes (Aniento et al. 1993), and apical transport vesicles in epithelial cells (Lafont et al. 1995). Mutational analysis in yeast, Neurospora, and...