Mutants at the Slender1 Locus of Barley cv Himalaya. Molecular and Physiological Characterization (original) (raw)
Journal Article
1Commonwealth Scientific and Industrial Research Organization, Plant Industry, G.P.O. Box 1600, Canberra, Australian Capitol Territory 2601, Australia (P.M.C., A.M.-P., M.E., F.G.); and
Search for other works by this author on:
1Commonwealth Scientific and Industrial Research Organization, Plant Industry, G.P.O. Box 1600, Canberra, Australian Capitol Territory 2601, Australia (P.M.C., A.M.-P., M.E., F.G.); and
2Laboratory of Seed Biology, Institut National de la Recherche Agronomique, 78026 Versailles cedex, France (A.M.-P.)
Search for other works by this author on:
1Commonwealth Scientific and Industrial Research Organization, Plant Industry, G.P.O. Box 1600, Canberra, Australian Capitol Territory 2601, Australia (P.M.C., A.M.-P., M.E., F.G.); and
Search for other works by this author on:
1Commonwealth Scientific and Industrial Research Organization, Plant Industry, G.P.O. Box 1600, Canberra, Australian Capitol Territory 2601, Australia (P.M.C., A.M.-P., M.E., F.G.); and
Search for other works by this author on:
Received:
09 October 2001
Revision received:
15 November 2001
Accepted:
20 January 2002
Cite
Peter Michael Chandler, Annie Marion-Poll, Marc Ellis, Frank Gubler, Mutants at the Slender1 Locus of Barley cv Himalaya. Molecular and Physiological Characterization, Plant Physiology, Volume 129, Issue 1, May 2002, Pages 181–190, https://doi.org/10.1104/pp.010917
Close
Navbar Search Filter Mobile Enter search term Search
Abstract
A dominant dwarf mutant of barley (Hordeum vulgare) that resembles dominant gibberellin (GA) “-insensitive” or “-nonresponsive” mutants in other species is described. α-Amylase production by endosperm half-grains of the mutant required GA3 at concentrations about 100 times that of the WT. The mutant showed only a slight growth response to GA3, even at very high concentrations. However, when additionally dwarfed, growth rate responded to GA3over the normal concentration range, although only back to the original (dwarf) elongation rate. Genetic studies indicated that the dominant dwarf locus was either closely linked or identical to the_Sln1_ (Slender1) locus. A barley sequence related to Arabidopsis GAI/RGA was isolated, and shown to represent the Sln1 locus by the analysis of_sln1_ mutants. The dominant dwarf mutant was also altered in this sequence, indicating that it too is an allele at_Sln1_. Thus, mutations at Sln1 generate plants of radically different phenotypes; either dwarfs that are largely dominant and GA “-insensitive/-nonresponsive,” or the recessive slender types in which GA responses appear to be constitutive. Immunoblotting studies showed that in growing leaves, SLN1 protein localized almost exclusively to the leaf elongation zone. In mutants at the Sln1 locus, there were differences in both the abundance and distribution of SLN1 protein, and large changes in the amounts of bioactive GAs, and of their metabolic precursors and catabolites. These results suggest that there are dynamic interactions between SLN1 protein and GA content in determining leaf elongation rate.
Copyright © 2002 American Society of Plant Physiologists
This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open\_access/funder\_policies/chorus/standard\_publication\_model)
You do not currently have access to this article.
Personal account
- Sign in with email/username & password
- Get email alerts
- Save searches
- Purchase content
- Activate your purchase/trial code
- Add your ORCID iD
Get help with access
Institutional access
Access to content on Oxford Academic is often provided through institutional subscriptions and purchases. If you are a member of an institution with an active account, you may be able to access content in one of the following ways:
IP based access
Typically, access is provided across an institutional network to a range of IP addresses. This authentication occurs automatically, and it is not possible to sign out of an IP authenticated account.
Sign in through your institution
Choose this option to get remote access when outside your institution. Shibboleth/Open Athens technology is used to provide single sign-on between your institution's website and Oxford Academic.
- Click Sign in through your institution.
- Select your institution from the list provided, which will take you to your institution's website to sign in.
- When on the institution site, please use the credentials provided by your institution. Do not use an Oxford Academic personal account.
- Following successful sign in, you will be returned to Oxford Academic.
If your institution is not listed or you cannot sign in to your institution's website, please contact your librarian or administrator.
Sign in with a library card
Enter your library card number to sign in. If you cannot sign in, please contact your librarian.
Society Members
Society member access to a journal is achieved in one of the following ways:
Sign in through society site
Many societies offer single sign-on between the society website and Oxford Academic. If you see "Sign in through society site" in the sign in pane within a journal:
- Click Sign in through society site.
- When on the society site, please use the credentials provided by that society. Do not use an Oxford Academic personal account.
- Following successful sign in, you will be returned to Oxford Academic.
If you do not have a society account or have forgotten your username or password, please contact your society.
Sign in using a personal account
Some societies use Oxford Academic personal accounts to provide access to their members. See below.
Personal account
A personal account can be used to get email alerts, save searches, purchase content, and activate subscriptions.
Some societies use Oxford Academic personal accounts to provide access to their members.
Viewing your signed in accounts
Click the account icon in the top right to:
- View your signed in personal account and access account management features.
- View the institutional accounts that are providing access.
Signed in but can't access content
Oxford Academic is home to a wide variety of products. The institutional subscription may not cover the content that you are trying to access. If you believe you should have access to that content, please contact your librarian.
Institutional account management
For librarians and administrators, your personal account also provides access to institutional account management. Here you will find options to view and activate subscriptions, manage institutional settings and access options, access usage statistics, and more.
Purchase
Short-term Access
To purchase short-term access, please sign in to your personal account above.
Don't already have a personal account? Register
Mutants at the Slender1 Locus of Barley cv Himalaya. Molecular and Physiological Characterization - 24 Hours access
EUR €39.00
GBP £33.00
USD $43.00
Rental
This article is also available for rental through DeepDyve.