A Polyamine Metabolon Involving Aminopropyl Transferase Complexes in Arabidopsis (original) (raw)
Journal Article
aUnitat de Fisiologia Vegetal, Facultat de Farmàcia, Universitat de Barcelona, Diagonal 643, 08028-Barcelona, Spain
Search for other works by this author on:
bInstituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia–Consejo Superior de Investigaciones Científicas, Avda de los Naranjos s/n, 46022 Valencia, Spain
Search for other works by this author on:
cMax-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, D-50829 Köln, Germany
Search for other works by this author on:
aUnitat de Fisiologia Vegetal, Facultat de Farmàcia, Universitat de Barcelona, Diagonal 643, 08028-Barcelona, Spain
Search for other works by this author on:
bInstituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia–Consejo Superior de Investigaciones Científicas, Avda de los Naranjos s/n, 46022 Valencia, Spain
Search for other works by this author on:
bInstituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia–Consejo Superior de Investigaciones Científicas, Avda de los Naranjos s/n, 46022 Valencia, Spain
Search for other works by this author on:
aUnitat de Fisiologia Vegetal, Facultat de Farmàcia, Universitat de Barcelona, Diagonal 643, 08028-Barcelona, Spain
Search for other works by this author on:
cMax-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, D-50829 Köln, Germany
Search for other works by this author on:
aUnitat de Fisiologia Vegetal, Facultat de Farmàcia, Universitat de Barcelona, Diagonal 643, 08028-Barcelona, Spain
Search for other works by this author on:
Published:
01 October 2002
Cite
Mireia Panicot, Eugenio G. Minguet, Alejandro Ferrando, Rubén Alcázar, Miguel A. Blázquez, Juan Carbonell, Teresa Altabella, Csaba Koncz, Antonio F. Tiburcio, A Polyamine Metabolon Involving Aminopropyl Transferase Complexes in Arabidopsis , The Plant Cell, Volume 14, Issue 10, October 2002, Pages 2539–2551, https://doi.org/10.1105/tpc.004077
Close
Navbar Search Filter Mobile Enter search term Search
Abstract
The conversion of putrescine to spermidine in the biosynthetic pathway of plant polyamines is catalyzed by two closely related spermidine synthases, SPDS1 and SPDS2, in Arabidopsis. In the yeast two-hybrid system, SPDS2 was found to interact with SPDS1 and a novel protein, SPMS (spermine synthase), which is homologous with SPDS2 and SPDS1. SPMS interacts with both SPDS1 and SPDS2 in yeast and in vitro. Unlike SPDS1 and SPDS2, SPMS failed to suppress the spe_Δ_3 deficiency of spermidine synthase in yeast. However, SPMS was able to complement the spe_Δ_4 spermine deficiency in yeast, indicating that SPMS is a novel spermine synthase. The SPDS and SPMS proteins showed no homodimerization but formed heterodimers in vitro. Pairwise coexpression of hemagglutinin- and c-Myc epitope–labeled proteins in Arabidopsis cells confirmed the existence of coimmunoprecipitating SPDS1-SPDS2 and SDPS2-SPMS heterodimers in vivo. The epitope-labeled SPDS and SPMS proteins copurified with protein complexes ranging in size from 650 to 750 kD. Our data demonstrate the existence of a metabolon involving at least the last two steps of polyamine biosynthesis in Arabidopsis.
© 2002 American Society of Plant Biologists
This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model ( https://academic.oup.com/journals/pages/open\_access/funder\_policies/chorus/standard\_publication\_model)
You do not currently have access to this article.
Personal account
- Sign in with email/username & password
- Get email alerts
- Save searches
- Purchase content
- Activate your purchase/trial code
- Add your ORCID iD
Get help with access
Institutional access
Access to content on Oxford Academic is often provided through institutional subscriptions and purchases. If you are a member of an institution with an active account, you may be able to access content in one of the following ways:
IP based access
Typically, access is provided across an institutional network to a range of IP addresses. This authentication occurs automatically, and it is not possible to sign out of an IP authenticated account.
Sign in through your institution
Choose this option to get remote access when outside your institution. Shibboleth/Open Athens technology is used to provide single sign-on between your institution’s website and Oxford Academic.
- Click Sign in through your institution.
- Select your institution from the list provided, which will take you to your institution's website to sign in.
- When on the institution site, please use the credentials provided by your institution. Do not use an Oxford Academic personal account.
- Following successful sign in, you will be returned to Oxford Academic.
If your institution is not listed or you cannot sign in to your institution’s website, please contact your librarian or administrator.
Sign in with a library card
Enter your library card number to sign in. If you cannot sign in, please contact your librarian.
Society Members
Society member access to a journal is achieved in one of the following ways:
Sign in through society site
Many societies offer single sign-on between the society website and Oxford Academic. If you see ‘Sign in through society site’ in the sign in pane within a journal:
- Click Sign in through society site.
- When on the society site, please use the credentials provided by that society. Do not use an Oxford Academic personal account.
- Following successful sign in, you will be returned to Oxford Academic.
If you do not have a society account or have forgotten your username or password, please contact your society.
Sign in using a personal account
Some societies use Oxford Academic personal accounts to provide access to their members. See below.
Personal account
A personal account can be used to get email alerts, save searches, purchase content, and activate subscriptions.
Some societies use Oxford Academic personal accounts to provide access to their members.
Viewing your signed in accounts
Click the account icon in the top right to:
- View your signed in personal account and access account management features.
- View the institutional accounts that are providing access.
Signed in but can't access content
Oxford Academic is home to a wide variety of products. The institutional subscription may not cover the content that you are trying to access. If you believe you should have access to that content, please contact your librarian.
Institutional account management
For librarians and administrators, your personal account also provides access to institutional account management. Here you will find options to view and activate subscriptions, manage institutional settings and access options, access usage statistics, and more.
Purchase
Short-term Access
To purchase short-term access, please sign in to your personal account above.
Don't already have a personal account? Register
A Polyamine Metabolon Involving Aminopropyl Transferase Complexes in Arabidopsis - 24 Hours access
EUR €39.00
GBP £33.00
USD $43.00
Rental
This article is also available for rental through DeepDyve.