High-Flavonol Tomatoes Resulting from the Heterologous Expression of the Maize Transcription Factor Genes LC and C1 (original) (raw)

Journal Article

,

aPlant Research International, Droevendaalsesteeg 1, P.O. Box 16, 6700 AA Wageningen, The Netherlands

Search for other works by this author on:

,

aPlant Research International, Droevendaalsesteeg 1, P.O. Box 16, 6700 AA Wageningen, The Netherlands

Search for other works by this author on:

,

aPlant Research International, Droevendaalsesteeg 1, P.O. Box 16, 6700 AA Wageningen, The Netherlands

Search for other works by this author on:

,

aPlant Research International, Droevendaalsesteeg 1, P.O. Box 16, 6700 AA Wageningen, The Netherlands

Search for other works by this author on:

,

aPlant Research International, Droevendaalsesteeg 1, P.O. Box 16, 6700 AA Wageningen, The Netherlands

Search for other works by this author on:

,

bUnilever Research, Colworth House, Sharnbrook, Bedford 44 MK 1LQ, United Kingdom

Search for other works by this author on:

,

bUnilever Research, Colworth House, Sharnbrook, Bedford 44 MK 1LQ, United Kingdom

Search for other works by this author on:

,

bUnilever Research, Colworth House, Sharnbrook, Bedford 44 MK 1LQ, United Kingdom

Search for other works by this author on:

,

bUnilever Research, Colworth House, Sharnbrook, Bedford 44 MK 1LQ, United Kingdom

Search for other works by this author on:

,

cUniversity of Exeter, School of Biological Sciences, Washington Singer Laboratory, Exeter EX4 4QG, United Kingdom

Search for other works by this author on:

... Show more

Published:

01 October 2002

• • Article contents
  • Figures & tables
  • Video
  • Audio
  • Supplementary Data
• Cite

Cite

Arnaud Bovy, Ric de Vos, Mark Kemper, Elio Schijlen, Maria Almenar Pertejo, Shelagh Muir, Geoff Collins, Sue Robinson, Martine Verhoeyen, Steve Hughes, Celestino Santos-Buelga, Arjen van Tunen, High-Flavonol Tomatoes Resulting from the Heterologous Expression of the Maize Transcription Factor Genes LC and C1, The Plant Cell, Volume 14, Issue 10, October 2002, Pages 2509–2526, https://doi.org/10.1105/tpc.004218
Close

Navbar Search Filter Mobile Enter search term Search

Abstract

Flavonoids are a group of polyphenolic plant secondary metabolites important for plant biology and human nutrition. In particular flavonols are potent antioxidants, and their dietary intake is correlated with a reduced risk of cardiovascular diseases. Tomato fruit contain only in their peel small amounts of flavonoids, mainly naringenin chalcone and the flavonol rutin, a quercetin glycoside. To increase flavonoid levels in tomato, we expressed the maize transcription factor genes LC and C1 in the fruit of genetically modified tomato plants. Expression of both genes was required and sufficient to upregulate the flavonoid pathway in tomato fruit flesh, a tissue that normally does not produce any flavonoids. These fruit accumulated high levels of the flavonol kaempferol and, to a lesser extent, the flavanone naringenin in their flesh. All flavonoids detected were present as glycosides. Anthocyanins, previously reported to accumulate upon LC expression in several plant species, were present in LC/C1 tomato leaves but could not be detected in ripe LC/C1 fruit. RNA expression analysis of ripening fruit revealed that, with the exception of chalcone isomerase, all of the structural genes required for the production of kaempferol-type flavonols and pelargonidin-type anthocyanins were induced strongly by the LC/C1 transcription factors. Expression of the genes encoding flavanone-3′-hydroxylase and flavanone-3′5′-hydroxylase, which are required for the modification of B-ring hydroxylation patterns, was not affected by LC/C1. Comparison of flavonoid profiles and gene expression data between tomato leaves and fruit indicates that the absence of anthocyanins in LC/C1 fruit is attributable primarily to an insufficient expression of the gene encoding flavanone-3′5′-hydroxylase, in combination with a strong preference of the tomato dihydroflavonol reductase enzyme to use the flavanone-3′5′-hydroxylase reaction product dihydromyricetin as a substrate.

© 2002 American Society of Plant Biologists

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model ( https://academic.oup.com/journals/pages/open\_access/funder\_policies/chorus/standard\_publication\_model)

You do not currently have access to this article.

Personal account

• Sign in with email/username & password
• Get email alerts
• Save searches
• Purchase content
• Activate your purchase/trial code
• Add your ORCID iD

Get help with access

Institutional access

Access to content on Oxford Academic is often provided through institutional subscriptions and purchases. If you are a member of an institution with an active account, you may be able to access content in one of the following ways:

IP based access

Typically, access is provided across an institutional network to a range of IP addresses. This authentication occurs automatically, and it is not possible to sign out of an IP authenticated account.

Sign in through your institution

Choose this option to get remote access when outside your institution. Shibboleth/Open Athens technology is used to provide single sign-on between your institution’s website and Oxford Academic.

1. Click Sign in through your institution.
2. Select your institution from the list provided, which will take you to your institution's website to sign in.
3. When on the institution site, please use the credentials provided by your institution. Do not use an Oxford Academic personal account.
4. Following successful sign in, you will be returned to Oxford Academic.

If your institution is not listed or you cannot sign in to your institution’s website, please contact your librarian or administrator.

Sign in with a library card

Enter your library card number to sign in. If you cannot sign in, please contact your librarian.

Society Members

Society member access to a journal is achieved in one of the following ways:

Sign in through society site

Many societies offer single sign-on between the society website and Oxford Academic. If you see ‘Sign in through society site’ in the sign in pane within a journal:

1. Click Sign in through society site.
2. When on the society site, please use the credentials provided by that society. Do not use an Oxford Academic personal account.
3. Following successful sign in, you will be returned to Oxford Academic.

If you do not have a society account or have forgotten your username or password, please contact your society.

Sign in using a personal account

Some societies use Oxford Academic personal accounts to provide access to their members. See below.

Personal account

A personal account can be used to get email alerts, save searches, purchase content, and activate subscriptions.

Some societies use Oxford Academic personal accounts to provide access to their members.

Viewing your signed in accounts

Click the account icon in the top right to:

• View your signed in personal account and access account management features.
• View the institutional accounts that are providing access.

Signed in but can't access content

Oxford Academic is home to a wide variety of products. The institutional subscription may not cover the content that you are trying to access. If you believe you should have access to that content, please contact your librarian.

Institutional account management

For librarians and administrators, your personal account also provides access to institutional account management. Here you will find options to view and activate subscriptions, manage institutional settings and access options, access usage statistics, and more.

Purchase

Short-term Access

To purchase short-term access, please sign in to your personal account above.

Don't already have a personal account? Register

High-Flavonol Tomatoes Resulting from the Heterologous Expression of the Maize Transcription Factor Genes LC and C1 - 24 Hours access

EUR €38.00

GBP £33.00

USD $41.00

Rental

This article is also available for rental through DeepDyve.

Citations

Views

Altmetric

Metrics

Total Views 1,713

1,258 Pageviews

455 PDF Downloads

Since 2/1/2021

Citations

369 Web of Science

×

Email alerts

Citing articles via

• Latest

• Most Read

• Most Cited

More from Oxford Academic