Cloning of the y1 Locus of Maize, a Gene Involved in the Biosynthesis of Carotenoids. (original) (raw)
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Department of Genetics, Iowa State University, Ames, Iowa 50011.
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Department of Genetics, Iowa State University, Ames, Iowa 50011.
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Department of Genetics, Iowa State University, Ames, Iowa 50011.
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Published:
01 September 1990
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B. Buckner, T. L. Kelson, D. S. Robertson, Cloning of the y1 Locus of Maize, a Gene Involved in the Biosynthesis of Carotenoids., The Plant Cell, Volume 2, Issue 9, September 1990, Pages 867–876, https://doi.org/10.1105/tpc.2.9.867
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Abstract
The y1 gene is one of the genes responsible for the production of [beta]-carotene in the endosperm and leaves of maize. We have cloned a Robertson's Mutator-tagged allele of the y1 gene (y1-mum) by using a Mu3 element as a hybridization probe. We substantiate that the cloned sequence is a portion of the y1 gene by molecular analyses of a revertant of a putative Mutator-induced y1 allele and the incidence of insertions within the cloned y1 sequence from several independently derived Mutator-induced y1 mutant stocks. The y1-mum sequence was used to isolate the standard Y1 allele, which conditions the presence of [beta]-carotene in the endosperm of the maize kernel.
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© 1990 by American Society of Plant Biologists
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