Isolation and characterization of migratory human skin dendritic cells (original) (raw)
Journal Article
,
Department of Cell Biology, Faculty of Medicine, Vrije Universiteit
, Amsterdam,
The Netherlands
Correspondence: C.D. Richters, Department of Cell Biology, Division of Electron Microscopy, Medical Faculty, Vrije Universiteit, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
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Euro Skin Bank, Research Department
, Beverwijk,
The Netherlands
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Euro Skin Bank, Research Department
, Beverwijk,
The Netherlands
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Euro Skin Bank, Research Department
, Beverwijk,
The Netherlands
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,
Department of Cell Biology, Faculty of Medicine, Vrije Universiteit
, Amsterdam,
The Netherlands
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Department of Cell Biology, Faculty of Medicine, Vrije Universiteit
, Amsterdam,
The Netherlands
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Cite
C D RICHTERS, M J HOEKSTRA, J VAN BAARE, J S DU PONT, E C M HOEFSMIT, E W A KAMPERDIJK, Isolation and characterization of migratory human skin dendritic cells, Clinical and Experimental Immunology, Volume 98, Issue 2, November 1994, Pages 330–336, https://doi.org/10.1111/j.1365-2249.1994.tb06146.x
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SUMMARY
A method is described to isolate and characterize human skin dendritic cells (DC). This method is based on the migratory capacities of these cells. The cells migrated “spontaneously” out of split-skin explants into the medium during a 24-h culture period and contained up to 75% CD1a+ cells. After removal of co-migrated T cells and macrophages, the highly enriched (>95% CD1a*) DC showed potent allo-antigen-presenting capacities. About 25% of the CD1a+ cells were also positive for the dermal DC marker CD1b, whereas only 15-20% of the cells contained Birbeck granules, the characteristic cell organelle of the epidermal Langerhans cell. Before culture. CD1a* DC were observed on cryostat sections not only in the epidermis but also in the dermis. After culture, the number of CD1a+ cells in both epidermis and dermis had decreased. Not all the cells had migrated during the culture period; some CD1a+ cells could still be detected in the epidermis and dermis after culture. Thus, using this method, potent allo-stimulating CD1a+ cells, migrating from both epidermis and dermis can be obtained without the use of enzymes.
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