Nucleotide sequence analysis and heterologous expression of the Erysipelothrix rhusiopathiae dnaJ gene (original) (raw)

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School of Biological Science, University of Nebraska, Lincoln, Nebraska, USA

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School of Biological Science, University of Nebraska, Lincoln, Nebraska, USA

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School of Biological Science, University of Nebraska, Lincoln, Nebraska, USA

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School of Biological Science, University of Nebraska, Lincoln, Nebraska, USA

Correspondence to: P. Blum, School of Biological Sciences, University of Nebraska, Lincoln, NE 68588-0118, USA.

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Present address: Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588, USA.

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David Rockabrand, Jim Partridge, Julie Krska, Paul Blum, Nucleotide sequence analysis and heterologous expression of the Erysipelothrix rhusiopathiae dnaJ gene, FEMS Microbiology Letters, Volume 111, Issue 1, July 1993, Pages 79–85, https://doi.org/10.1111/j.1574-6968.1993.tb06365.x
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Abstract

DNA sequence analysis of chromosomal DNA from the Gram-positive facultative intracellular pathogen, Erysipelothrix rhusiopathiae has identified a dnaJ heat shock gene homolog. A 1109-bp open reading frame encoding dnaJ is located immediately 3′ to the E. rhusiopathiae dnaK gene. The deduced DnaJ amino acid sequence exhibits the modular structure of other members of the DnaJ protein class including a glycine-rich region and the repeating consensus sequence CXXCXGXGX. Heterologous expression of the dnaJ sequence in Escherichia coli resulted in accumulation of a unique 38.9-kDa protein with an isoelectric point of 8.0. Deletion analysis of the dnaJ gene was used to confirm that the overproduced protein was encoded by the dnaJ sequence.

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Author notes

1

Present address: Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588, USA.

© 1993 Federation of European Microbiological Societies

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