INFERENCE OF SPERM COMPETITION FROM BROODS OF FIELD‐CAUGHT DROSOPHILA (original) (raw)
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Department of Biological Sciences University of Nebraska Lincoln Nebraska 68588
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Department of Biology Pennsylvania State University, University Park Pennsylvania 16802
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Published:
01 October 1998
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Abstract
In field studies of multiple mating and sperm competition there typically is no experimental control over the number of times that a female mates, the interval between matings, or the genetic identity of multiple fathers contributing to a brood. Irrespective of this complexity, high‐resolution molecular markers can be used to assign paternity with considerable confidence. This study employed two highly heterozygous microsatellite loci to assess multiple paternity and sperm displacement in a sample of broods taken from a natural population of Drosophila melanogaster. The large number of alleles present at each of the loci makes it difficult to derive explicit maximum‐likelihood estimates for multiple paternity and sperm displacement from brood samples. Monte Carlo simulations were used to estimate maximum‐likelihood parameters for the distribution of female remating frequency and the proportion of offspring sired by the second or subsequent mating males. Estimates were made based on genotypes scored at two distinct marker loci because they were found to give statistically homogeneous results. Fitting a Poisson distribution of number of matings, the mean number of males mated by a female was 1.82. The sperm displacement parameter estimated from doubly mated females were 0.79 and 0.86 for the two loci (0.83 for the joint estimate). The overall probability that a multiply mated female will be misclassified as singly mated was only 0.006, which indicates that microsatellites can provide excellent resolution for identifying multiple mating. In addition, microsatellites can be used to generate relatively precise estimates of sperm precedence in brood‐structured samples from a natural population.
Literature Cited
Anderson
,
W. W.
1974
.
Frequent multiple insemination in a natural population of Drosophila pseudoobscura.
Am. Nat.
108
:
709
–
711
.
Birkhead
,
T. R.
, and
F. F.
Hunter
.
1990
.
Mechanisms of sperm competition.
Trends Ecol. Evol.
5
:
48
–
52
.
Boorman
,
E.
, and
G. A.
Parker
.
1976
.
Sperm (ejaculate) competition in D. melanogaster and the reproductive value of females to males in relation to female egg and mating status.
Ecol. Entomol.
1
:
145
–
155
.
Clark
,
A. G.
,
M.
Aguadé
,
T.
Prout
,
L. G.
Harshman
, and
C. H.
Langley
.
1995
.
Variation in sperm displacement and its association with accessory gland protein loci in Drosophila melanogaster.
Genetics
139
:
189
–
201
.
Clark
,
A. G.
, and
D. J.
Begun
.
1998
.
Female genotypes affect sperm displacement in Drosophila.
Genetics
. In press.
Cobbs
,
G.
1977
.
Multiple insemination and male sexual selection in natural populations of Drosophila pseudoobscura.
Am. Nat.
111
:
641
–
656
.
Dobzhansky
,
T.
,
B.
Spassky
, and
T.
Tidwell
.
1963
.
Genetics of natural populations. XXXII. Inbreeding and the mutational and balanced genetic loads in natural populations of Drosophila pseudoobscura.
Genetics
48
:
361
–
374
.
Goldstein
,
D. B.
, and
A. G.
Clark
.
1995
.
Microsatellite variation in North American populations of Drosophila melanogaster.
Nuc. Acids Res.
23
:
3882
–
3886
.
Griffiths
,
R. C.
,
S. W.
McKechnie
, and
J. A.
McKenzie
.
1982
.
Multiple mating and sperm displacement in natural populations of Drosophila melanogaster.
Theor. Appl. Genet.
62
:
89
–
96
.
Gromko
,
M. H.
, and
T. A.
Markow
.
1993
.
Courtship and remating in field populations of Drosophila.
Anim. Behav.
45
:
253
–
262
.
Gromko
,
M. H.
,
D. G.
Gilbert
, and
R. C.
Richmond
.
1984
.
Sperm transfer and use in the multiple mating system of Drosophila.
Pp.
372
–
427
in
R. L.
Smith
, ed. Sperm competition and the evolution of animal mating systems.
Academic Press
,
New York
.
Gwynne
,
D. T.
1984
.
Sexual selection and sexual differences in Mormon crickets (Orthoptera: Tettigoniidae, Anabrus simplex).
Evolution
38
:
1011
–
1022
.
Harshman
,
L. G.
, and
T.
Prout
.
1994
.
Sperm displacement without sperm transfer in Drosophila melanogaster.
Evolution
48
:
758
–
766
.
Hughes
,
K. A.
1997
.
Quantitative genetics of sperm precedence in Drosophila melanogaster.
Genetics
145
:
139
–
151
.
Imhof
,
M.
,
B.
Harr
,
G.
Brem
, and
C.
Schloetterer
.
1998
.
Multiple mating in wild Drosophila melanogaster revisited by microsatellite analysis.
Mol. Ecol.
In press.
Lagercrantz
,
U.
,
H.
Ellegren
, and
L.
Anderson
.
1993
.
The abundance of various polymorphic microsatellite motifs differs between plants and vertebrates.
Nuc. Acids Res.
21
:
1111
–
1115
.
Lefevre
,
G.
, and
V. B.
Jonsson
.
1962
.
Sperm transfer, storage, displacement, and utilization in D. melanogaster.
Genetics
47
:
1719
–
1736
.
Marks
,
R. W.
,
R. D.
Seager
, and
L. G.
Barr
.
1988
.
Local ecology and multiple mating in natural populations of Drosophila melanogaster.
Am. Nat.
131
:
918
–
923
.
Milkman
,
R.
, and
R. R.
Zeitler
.
1974
.
Concurrent multiple paternity in natural and laboratory populations of Drosophila melanogaster.
Genetics
78
:
1191
–
1193
.
Newport
,
M. A.
, and
M. H.
Gromko
.
1984
.
The effect of experimental design on female receptivity to remating and its impact on reproductive success in D. melanogaster.
Evolution
38
:
1261
–
1272
.
Parker
,
G. A.
1970
.
Sperm competition and its evolutionary consequences in the insects.
Biol. Rev.
45
:
525
–
567
.
Parker
,
G. A.
1990
.
Analysing sperm competition data: simple models for predicting mechanisms.
Behav. Ecol. Sociobiol.
27
:
55
–
65
.
Parker
,
G. A.
1993
.
Sperm competition games: sperm size and sperm number under adult control.
Proc. R. Soc. Lond. B
253
:
245
–
254
.
Press
,
W. H.
,
S. A.
Teukolsky
,
W. T.
Vetterling
, and
B. P.
Flannery
.
1992
. Numerical recipes: the art of scecientific computing. 2d ed.
Cambridge Univ. Press
,
Cambridge, U.K
.
Prout
,
T.
, and
A. G.
Clark
.
1996
.
Polymorphism in genes that influence sperm displacement.
Genetics
144
:
401
–
408
.
Richmond
,
R. C.
,
D. G.
Gilbert
, and
K. B.
Sheehan
.
1980
.
Esterase 6 and reproduction in Drosophila melanogaster.
Science
207
:
1483
–
1485
.
Shug
,
M. D.
,
K. A.
Wetterstrand
,
M. S.
Gaudette
,
R. H.
Lim
,
C. M.
Hutter
, and
C. F.
Aquadro
.
1998
.
The distribution and frequency of microsatellite loci in Drosophila melanogaster.
Mol. Ecol.
7
:
57
–
70
.
Stalker
,
H. D.
1976
.
Chromosome studies in wild populations of Drosophila melanogaster.
Genetics
82
:
323
–
347
.
Weber
,
J. L.
, and
P. E.
May
.
1989
.
Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction.
Am. J. Hum. Genet.
44
:
388
–
396
.
Williams
,
C. J.
, and
S.
Evarts
.
1989
.
The estimation of concurrent paternity probabilities in natural populations.
Theor. Popul. Biol.
35
:
90
–
112
.
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