Peroxide Is a Key Mediator of Bcl-2 Down-Regulation and Apoptosis Induction by Cisplatin in Human Lung Cancer Cells (original) (raw)

Research ArticleArticle

, Pithi Chanvorachote, David Toledo, Christian Stehlik, Robert R. Mercer, Vincent Castranova and Yon Rojanasakul

Molecular Pharmacology January 2008, 73 (1) 119-127; DOI: https://doi.org/10.1124/mol.107.040873

Footnotes

• This work was supported by the National Institutes of Health grants HL071545 and HL076340 (to Y.R.).

• ABBREVIATIONS: cisplatin, _cis_-diamminedichloroplatinum; ROS, reactive oxygen species; ESR, electron spin resonance; GPx, glutathione peroxidase; CAT, catalase; SOD, superoxide dismutase; DMPO, 5,5-dimethyl-1-pyrroline-_N_-oxide; DCFH-DA, 2′,7′-dichlorodihydro-fluorescein diacetate; DHE, dihydroethidine; PBS, phosphate-buffered saline; CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]propanesulfonate; ELISA, enzyme-linked immunosorbent assay; AMC, amino-4-methyl coumarin; z-LEHD-fmk, _N_-benzyloxycarbonyl-Leu-Glu-His-Asp-fluoromethyl ketone; z-IETD-fmk, _N_-benzyloxycarbonyl-Ile-Glu-Thr-Asp-fluoromethyl ketone.

• • Received August 13, 2007.
  • Accepted October 2, 2007.

• The American Society for Pharmacology and Experimental Therapeutics

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